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Neutral Detergent Fiber (NDF) And Hemicellulose - Reflux Method


This procedure is used for the determination of amylase-treated neutral detergent fiber (NDF) in feed materials using a neutral detergent solution and heat. Sodium sulfite is used in the procedure to aid in the removal of some nitrogenous matter. Heat-stable amylase is used in the procedure to allow for the removal of starch and to inactivate potential contaminating enzymes that might degrade fibrous constituents. Hemicellulose is determined by performing the NDF procedure followed by the ADF procedure (see SOP 640 for ADF details). The detection limit for NDF and hemicellulose are approximately 0.5 %, and 1.0 % respectively. On homogenous sample material, the method is generally reproducible within 8% (relative). Results for NDF can be reported on an ash-free basis upon client request.

Sample amount requested:  10 g

Questions concerning limited sample size can be answered by the UC Davis Analytical Laboratory.

AOAC Official Method 2002-04, Amylase-Treated Neutral Detergent Fiber in Feeds, Using Refluxing in Beakers or Crucibles, in Official Methods of Analysis of AOAC International, (2006), Chapter 4, pp. 48-55, AOAC International, Arlington, VA.