You are here: Home / Using the Lab / Analysis / Olive Oil / 972

972

Sterols and Triterpene Dialcohols in Oil by Gas Chromatography

The method describes a procedure for determining the individual and total sterols and triterpene dialcohols content of olive oils and olive pomace oils. The oils are saponified with ethanolic potassium and the unsaponifiable matter is extracted with ether. The sterols and triterpene dialcohols fraction are separated from the unsaponifiable matter by silica gel chromatography. The fractions recovered from the silica gel are derivatized into trimethylsilyl ethers and analyzed by capillary column gas chromatography with a flame ionization detector.

Reference: Determination of the Composition and Content of Sterols and Triterpene Dialcohols by Capillary Column Gas Chromatography, International Olive Council, COI/ T.20/ Doc. no. 30, 2009.