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Wax Content in Oil by Gas Chromatography

Determination of the content of waxes, fatty acid methyl and ethyl esters in olive oils by gas chromatography. The waxes are purified on a silica gel column and analyzed by gas chromatography with a flame ionization detector.  The individual waxes and alkyl esters are separated according to the number of carbon atoms. The method is recommended as a tool for distinguishing between olive oil and olive-pomace oil and as a quality parameter for extra virgin olive oils enabling the detection of fraudulent mixtures of extra virgin olive oils with lower quality oils whether they are virgin, ordinary, lampante or some deodorised oils.

Reference: Determination of the Content of Waxes, Fatty Acid Methyl Esters and Fatty Acid Ethyl Esters by Capillary Gas Chromatography, International Olive Council,
COI/T.20/Doc. No 28/Rev. 1, 2010