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Stigmastadiene in Oil by Gas Chromatography

The method describes a procedure for determining the stigmastadiene content of vegetable oils. The oils are saponified with ethanolic potassium and the unsaponifiable matter is extracted with hexane. The stigmastadiene fraction is separated from the unsaponifiable matter by silica gel chromatography. The fraction recovered from the silica gel is analyzed by capillary column gas chromatography. The method is particularly suited to detecting the presence of refined vegetable oils (olive, olive-pomace, sunflower, soybean, palm, etc) in virgin olive oil since refined oils contain Stigmastadienes and virgin oils do not.

Reference: Determination of Stigmastadienes in Vegetable Oils, International Olive Council, COI/T.20/Doc. no. 11/Rev. 2, 2001