This analytical method quantitatively determines the total amount of nitrogen and carbon in botanical material using an instrument that utilizes a combustion system with an induction furnace coupled with a thermal conductivity detector (TCD) system and an IR detector system. The method is based on the oxidation of the sample by “flash combustion” which converts all organic and inorganic substances into combustion gases (N2, NOx, CO2, and H2O). The method has a detection limit of approximately 0.02% for nitrogen and 0.1% for carbon.
Sample amount requested: 3 g (for either or both)
Questions concerning limited sample size can be answered by the UC Davis Analytical Laboratory.
AOAC Official Method 972.43, Microchemical Determination of Carbon, Hydrogen, and Nitrogen, Automated Method, in Official Methods of Analysis of AOAC International, 18th edition, Revision 1, 2006. Chapter 12, pp. 5-6, AOAC International, Gaithersburg, MD.
AOAC Official Method 990.03. Protein (Crude) in Animal Feed, Combustion Method, in Official Methods of Analysis of AOAC International, 18th Edition (2005). Revision 1, 2006, Chapter 4, pp. 30-31. AOAC International, Arlington, VA.
